Characterization of the antibody response against EV71 capsid proteins in Chinese individuals by NEIBM-ELISA

نویسندگان

  • Yingying Ding
  • Xuguang Chen
  • Baohua Qian
  • Guorong Wu
  • Ting He
  • Jiaojiao Feng
  • Caixia Gao
  • Lili Wang
  • Jinhong Wang
  • Xiangyu Li
  • Mingmei Cao
  • Heng Peng
  • Chunyan Zhao
  • Wei Pan
چکیده

Human enterovirus 71 (EV71) has become the major pathogen of hand, foot, and mouth disease (HFMD) worldwide, while the anti-EV71 antibody responses other than neutralizing epitopes have not been characterized. In this study, EV71 capsid proteins VP1, VP3, VP0 and various VP1 antigens were constructed to analyze anti-EV71 response in severe HFMD cases, non-HFMD outpatient children and normal adults using a novel evolved immunoglobulin-binding molecule (NEIBM)-based ELISA. The high prevalence of antibody responses against all three capsid proteins was demonstrated, and anti-EV71 VP1 showed the main antibody response. Anti-EV71 VP1 antibody response was found to predominantly target to epitopes based on the common enterovirus cross-reactive sequence. Moreover, inhibition pattern against anti-EV71 VP1 reactions in three groups was obviously different. Taken together, these results firstly characterized the anti-EV71 antibody responses which are predominantly against VP1 epitopes based on common enterovirus cross-reactive sequence. This finding could be helpful for the better understanding of anti-EV71 humoral immunity and useful for seroepidemiological surveillance.

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عنوان ژورنال:

دوره 5  شماره 

صفحات  -

تاریخ انتشار 2015